Welcome to our Downstream Processing Library.

This library contains citations for our publications in the field of downstream processing. Copyright restrictions preclude us from providing electronic copies for some entries, but you are welcome to download any linked files for your individual personal used. Entries are posted in reverse chronological order in each section. There is a lot of new material and we’ll be updating content regularly so feel free to visit as often as you like. New entries are prepared in Acrobat 7. If you need to update your copy of Acrobat Reader, you can download a free copy from http://www.adobe.com/.

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Publications on hydroxyapatite
Morrison, C. J., Gagnon, P., Cramer, S. M., Unique selectivity windows using selective displacers/eluents and mobile phase modifiers on hydroxyapatite, J.Chromatogr. A 1217 6484-6495

Gagnon, P., 2010, Dissociation of antibody-contaminant complexes with hydroxyapatite, Bioprocessing J., 9(4) 14-24

Gagnon, P., Dissociation of naturally occuring antibody-contaminant complexes with hydroxyapatite, Oral presentation, Bioprocess International Conference, Providence, September 20-24, 2010

Gagnon, P., Hensel, F., Lee, S., Zaidi, S., 2010, IgM Purification: The Fine Print, Oral presentation, 4th International Monolith Symposium, Portoroz, Slovenia, May 29 - June 2, 2010

Gagnon, P., 2010, Hydroxyapatite for Biomolecule Purification, Genetic Engineering News, 30(7) 28-31

Gagnon, P., Cheung C -W., Lepin, E. J., Wu , A. M., Sherman. M. A., Raubitschek, A. A., Yazaki, P. J., Minibodies and Multimodal Chromatography Methods, A Convergence of Challenge and Opportunity, BioProcess International, 8(2) 2010 26-35

Gagnon, P., Cheung C .-W., Yazaki, P., Cooperative Multimodal Retention of IgG, Fragments, and Aggregates on Hydroxyapatite, Journal of Separation Science, 32, 2009, 3857-3865. A reprint may be requested at this link.

Snyder, M., Ng, P., Mekosh, H., Gagnon, P., PEG Enhances Viral Clearance on Ceramic Hydroxyapattie, Journal of Separation Science, 32, 2009, 4048-4051. A reprint may be requested from the first author.

Gagnon, P., Cheung, C-W., Yazaki, 2009, A Case Study of Early Phase Purification Process Development for an Anti-Cancer Minibody, 5th International Symposium on Hydroxyapatite, Rottach-Egern, Germany, October 11-14.

Hensel, F., Gagnon, P., 2009, An effective platform for purification of IgM monoclonal antibodies, 5th International Symposium on Hydroxyapatite, Rottach-Egern, Germany, October 11-14.

Gagnon, P., 2009, Multiple Options for Aggregate Removal by Apatite Chromatography, Oral presentation, BioProcess International China, Beijing, September 7-9.

Gagnon, P., Beam, K., 2009, Antibody Aggregate Removal with Hydroxyapatite, Current Pharmaceutical Biotechnology 10(4) 440-446

Gagnon, P., 2009, Monoclonal Antibody Purification with Hydroxyapatite, New Biotechnology, 25 287-293. The above link will tak you to the published version of the article on Science Direct. The final prepublication draft can be downloaded at this link.

Gagnon, P., Cheung, C-W., Yazaki, P., 2009, Multimodal Interactions of IgG, Fab, F(ab)2, Fc, and Aggregates with Hydroxyapatite, Poster, 22nd International IBC Antibody Development and Production, Carlsbad, CA, March 4-6

Gagnon, P., Cheung, C-W., Yazaki, P., 2009, Reverse Calcium Affinity Purification of Fab with Calcium Derivatized Hydroxyapatite, J. Immunol. Met., 342 115-118. The above link will tak you to the published version of the article on Science Direct. The final prepublication draft can be downloaded at this link.

Gagnon, P., Cheung, C-W., Yazaki, P., Brinkman, A., Aolin, F. (2008) New Insights into IgG Binding and Aggregate Removal with Hydroxyapatite, 8th International Ube Bioseparation Symposium, Nov. 15-19.

Gagnon, P., Cheung, C.-W., Yazaki, P. (2008) Multiple Modes of Fab Purification with Hydroxyapatite, BioProcess International Asia Pacific, Mumbai, October 20-22

Gagnon, P. (2008) Improved Antibody Aggregate Removal by Hydroxyapatite Chromatography in the Presence of Polyethylene Glycol, J. Immunol. Met., 336 222-228

Phosphate-free Buffer Systems: A New Frontier for Apatite Chromatography, 4th International Conference on Hydroxypatite, Sonoma, CA May 5-7, 2008

Multiple Modes of Aggregate Removal by Hydroxyapatite, 4th International Conference on Hydroxypatite, Sonoma, CA May 5-7, 2008

Purification of IgM Monoclonal Antibodies, 2008, Pete Gagnon, Frank Hensel, Richard Richieri, BioPharm Inernational, March suppl., 26-35

IgM Purification: The Next Generation, Pete Gagnon, Frank Hensel, Paul Andrews, Richard Richieri, Oral Presentation, 13th Waterside Monoclonal Conference, February 4-6, 2008, Miami.

Recent advances in the purification of IgM monoclonal antibodies, Pete Gagnon, Frank Hensel, Paul Andrews, Richard Richieri, Third Wilbio Conference on Purification of Biological Products, September 24-26, Waltham, MA USA.

Nonionic Polymer Enhancement of Aggregate Removal by Ion Exchange and Hydroxyapatite Chromatography, Pete Gagnon, Richard Rishieri, Simin Zaidi, 12th Annual Waterside Conference, San Juan, Puerto Rico, Apr 23-25, 2007. Oral presentation, pdf.

Buffers and conditions for use and maintenance of ceramic hydroxyapatite, Julia Zhen, Pete Gagnon, Mark Snyder, 18th International IBC Conference on Antibody Development and Production, La Costa Spa and Resort, Carlsbad, CA, Feb 28 - Mar 2, 2007. Poster PDF

Recent improvements in the use of hydroxyapatite for removal of aggregates from monoclonal antibodies. Pete Gagonon, Powerpoint PDF. Feb. 28, 2007.

A Mechanistic Model for Adsorption of Immunoglobulin on Hydroxyapatite, Paul Ng, Jie He, and Pete Gagnon, J. Chromatography A, 1142 (1) 13-28 (2007)

In the same issue, see also...
Comparison of ceramic hydroxy- and fluoroapatite versus Protein A/G-based resins in the isolation of a recombinant human antibody from cell culture supernatant, 2007, Sven Schubert and Ruth Freitag, J. Chromatography A, 1142 (1) 106-113 (2007)

Hydroxyapatite as a Capture Method for Purification of Monoclonal Antibodies, P. Gagnon, S. Zaidi, and S. Summers, IBC World Conference and Exposition, San Francisco, Nov. 6-9, 2006. Awarded Best Poster, Recovery and Purification.

Practical Issues in the Industrial use of Hydroxyapatite for Purification of Monoclonal Antibodies (Oral presentation, ACS, San Francisco, 9/10/06)

The effect of different Hofmeister ions on aggregate removal by hydroxyapatite (Poster presentation, ACS, San Francisco, 9/13/06)

P. Ng, A. Cohen, P. Gagnon, 2006, Monoclonal antibody purification with CHT, Genetic Engineering News, 26(14)

P. Gagnon, P. Ng, C. Aberrin, J. Zhen, J. He, H. Mekosh, L. Cummings, R. Richieri, S. Zaidi, 2006, A ceramic hydroxyapatite based purification platform: simultaneous removal of leached protein A, aggregates, DNA, and endotoxins, BioProcess International, 4(2) 50-60. Provision of this reprint was kindly permitted by BioProcess International. You can access this and other articles on their website at <http://www.bioprocessintl.com>

P. Ng, C. Aberin, J. He, H. Mekosh, J. Zhen and P. Gagnon, 2006, A 3-mechanism model for adsorption of IgG on CHT ceramic hydroxyapatite, Poster, Prep 2006, May 14-17, Baltimore, MD. Posted courtesy of Bio-Rad Laboratories.

P. Gagnon, P. Ng, J. He, J. Zhen, C. Aberrin, H. Mekosh, 2006, The practical task of purifying antibodies with ceramic hydroxyapatite, Oral presentation, 11^th Annual Waterside Conference, May 1-3, Chicago. Posted courtesy of Bio-Rad Laboratories.

P. Gagnon, P. Ng, J. He, J. Zhen, C. Aberrin, H. Mekosh, 2006, A tale of two platforms, 2-step purification of monoclonal IgG with protein A and ceramic hydroxyapatite, Oral presentation, IBC conference on production and purification of monoclonal antibodies, March 1-3, Carlsbad, CA. Posted courtesy of Bio-Rad Laboratories.

P. Gagnon, P. Ng, C. Aberrin, J. Zhen, J. He, H. Mekosh, L. Cummings, 2005, Simultaneous removal of aggregate, leached protein A, endotoxin, and DNA from protein A purified monoclonal IgG with ceramic hydroxyapatite and ceramic fluorapatite, Oral presentation, First Annual Conference on Purification of Biologics, Dec. 5-7, Santa Monica, CA. Posted courtesy of Bio-Rad Laboratories.

P. Ng, N. Liu, C. Eller, S. Farashchi, H. Chen, A. Paulus and P. Gagnon, 2005, Application of CHT™ Ceramic Hydroxyapatite for Proteomic Analysis, Poster, ISPPP: 25th International Symposium on the Separation of Proteins, Peptides, and Polynucleotides. Nov. 6-9, St. Petersburg, FL. Posted courtesy of Bio-Rad Laboratories.

P. Ng, P. Gagnon and C. Aberin, 2005, Differential retention of DNA on ceramic hydroxyapatite and ceramic fluorapatite, Poster, Prep 2005 May 2-6, Philadelphia, PA. Posted courtesy of Bio-Rad Laboratories.

P. Gagnon, P. Ng, C. Aberrin, J. Zhen, J. He, H. Mekosh, L. Cummings, 2005, Retention behavior of endotoxin, DNA, and protein A on ceramic hydroxyapatite and ceramic fluorapatite, Oral presentation, IBC World Conference, Sept. 14-16, Boston. Posted courtesy of Bio-Rad Laboratories.

P. Gagnon, 1998, An Enigma Unmasked: How Hydroxtapatite Works and How to Make it Work For You, http://www.validated.com/revalbio/pdffiles/hxyapt.pdf

P. Gagnon, 1997, Antibody purification with hydroxyaptatite chromatography: the basics and beyond, Oral presentation, 2nd Waterside Monoclonal Conference, Norfolk